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hyperforin in methanol  (Sequoia Research)

 
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    Sequoia Research hyperforin in methanol
    Hyperforin In Methanol, supplied by Sequoia Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hyperforin+in+methanol/hyperforin+in+methanol/pm19122289-42-0-8
    Average 90 stars, based on 1 article reviews
    hyperforin in methanol - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Inhibitory effects of herbal extracts on the activity of human sulfotransferase isoform sulfotransferase 1A3 (SULT1A3).
    Article Snippet: Hyperforin (1 mg/ml in methanol) was purchased from Sequoia Research Products Ltd. (Oxford, U.K.).



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    hyperforin in methanol  (Sequoia Research)
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    3T3-L1 preadipocytes, induced to differentiate using the typical MDI cocktail, were treated at the time of induction with DMSO vehicle (V), methanol vehicle (M), 50 μg/ml flower (F) and root (R) extracts of SJW, or the indicated the doses of hypericin (HI) and <t>hyperforin</t> (HF). The cells were retreated following each media change every 48–72 hours. Control cells (C) received no treatment. Each panel is representative of 3–6 independently performed experiments. A: Oil Red O staining of neutral lipid content was performed 7 days post-MDI. All of the wells shown were stained at the same time with the same Oil Red O working solution. B: Western blot analysis of whole cell extracts from cells harvested four days post-MDI. Similar results were obtained at 4 and 7 days post-MDI, and panel B contains a representative subset of these blots.
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    Average 90 stars, based on 1 article reviews
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    3T3-L1 preadipocytes, induced to differentiate using the typical MDI cocktail, were treated at the time of induction with DMSO vehicle (V), methanol vehicle (M), 50 μg/ml flower (F) and root (R) extracts of SJW, or the indicated the doses of hypericin (HI) and hyperforin (HF). The cells were retreated following each media change every 48–72 hours. Control cells (C) received no treatment. Each panel is representative of 3–6 independently performed experiments. A: Oil Red O staining of neutral lipid content was performed 7 days post-MDI. All of the wells shown were stained at the same time with the same Oil Red O working solution. B: Western blot analysis of whole cell extracts from cells harvested four days post-MDI. Similar results were obtained at 4 and 7 days post-MDI, and panel B contains a representative subset of these blots.

    Journal: Biochimica et Biophysica Acta

    Article Title: St. John's Wort inhibits insulin signaling in murine and human adipocytes

    doi: 10.1016/j.bbadis.2011.12.005

    Figure Lengend Snippet: 3T3-L1 preadipocytes, induced to differentiate using the typical MDI cocktail, were treated at the time of induction with DMSO vehicle (V), methanol vehicle (M), 50 μg/ml flower (F) and root (R) extracts of SJW, or the indicated the doses of hypericin (HI) and hyperforin (HF). The cells were retreated following each media change every 48–72 hours. Control cells (C) received no treatment. Each panel is representative of 3–6 independently performed experiments. A: Oil Red O staining of neutral lipid content was performed 7 days post-MDI. All of the wells shown were stained at the same time with the same Oil Red O working solution. B: Western blot analysis of whole cell extracts from cells harvested four days post-MDI. Similar results were obtained at 4 and 7 days post-MDI, and panel B contains a representative subset of these blots.

    Article Snippet: 2.1 Materials Hypericin and a methanolic solution of hyperforin, both isolated from St. John’s Wort, and Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Staining, Western Blot